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由 J Yang 著作2009被引用 42 次 — ... yeast Pichia pastoris, in which the Escherichia coli toxin gene mazF was used as a counter-selectable marker. mazF ...
由 J Yang 著作2009被引用 42 次 — ... and will be a useful tool for P. pastoris studies. Pichia pastoris, mazF, counter- selectable marker, genetic modification.
mazF was placed under the tightly controlled AOX1 promoter, and the induced expression of MazF in P. pastoris halted ...
The recombinant Pichia pastoris lays certain foundation for high-efficiency preparation of small-molecular ... Using the toxin gene mazF of Escherichia coli and blasticidin resistance gene Zeocin as two ...
... yeast Pichia pastoris, in which the Escherichia coli toxin gene mazF was used as a counter-selectable marker.
mazF as a counter-selectable marker for unmarked genetic modification of Pichia pastoris. Yang J, Jiang W, Yang S
... yeast Pichia pastoris, in which the Escherichia coli toxin gene mazF was used as a counter-selectable marker. mazF ...
由 C Li 著作2017被引用 13 次 — Pichia pastoris is a widely used heterologous protein production workhorse. ... the genome after each marker was rescued for knock-in of a gene of interest using mazF, and the toxicity ...
由 Z Chen 著作2013被引用 21 次 — The OCH1 gene of P. pastoris was chosen as an illustrative ... MazF toxicity causes a strong selection pressure on the streaked strains and induces the loss of the helper plasmids.

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