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At a wavelength of 260 nm, the average extinction coefficient for double-stranded DNA is 0.020 (μg/ml)1 cm1, for single-stranded DNA it is 0.027 (μg/ml)1 cm1, for single-stranded RNA it is 0.025 (μg/ml)1 cm1 and for short single-stranded oligonucleotides it is dependent on the length and base composition.
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coefficiency of dsdna
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The molar extinction coefficients for DNA are: 50 (μg/mL)-1cm-1 for double-stranded DNA (Absrobance max at 260 nm); 33 (μg/mL)-1cm-1 for ...
Aug 7, 2009Generally, these standard coefficients are used in place of the extinction coefficient for double stranded DNA (dsDNA), single stranded RNA, ...
6 pages·229 KB
Dec 27, 2020At a wavelength of 260 nm, the average extinction coefficient for double-stranded DNA is 0.020 (μg/ml)−1 cm−1, for single-stranded DNA it ...
This comparative shows that ssDNA was obtained from dsDNA, since single stranded DNA has a higher absorption coefficient than double stranded DNA. A solution of ...
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High degree of linearity from 0.1 to 100 µg/mL DNA · MARS evaluation software offers integrated extinction coefficients for dsDNA, ssDNA and RNA · Measure a full ...
Double stranded DNA: 50
Single stranded DNA: 33
RNA: 40
For example, for the tetranucleotide 5'-ACGT-3', there are three dinucleotides (AC, CG, and GT). The extinction coefficients for these pairs are 21,200, 18,000, ...
Double-stranded DNA ... concentration (ug/ml) = OD260 x conversion factor ... 1 OD260 Unit = 50 μg/ml for dsDNA; 1 OD260 Unit = 40 μg/ml ssRNA; 1 OD260 Unit ...
by AO Nwokeoji2017Cited by 40extinction coefficient (ε) of constituent nucleotides. However, ... extinction coefficients/hypochromicity of dsDNA, dsRNA and.

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